Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines

TL;DR — Key Findings

AP-PG induced several interferon-stimulated genes in macrophage-like cells.
Interferon and STAT1-related mechanisms both contributed.
The experiment does not demonstrate human antiviral efficacy.

Abstract

Researchers stimulated RAW264.7 and other macrophage-like cell models with Aureobasidium pullulans-produced beta-glucan (AP-PG). Mx1, ISG15, and viperin messenger RNA increased, IFN-beta rose transiently, and STAT1 Ser727 phosphorylation occurred rapidly. AP-PG also enhanced viperin induction by interferon-alpha, suggesting both interferon induction and strengthened STAT1-mediated transcription. These are mechanistic cell findings and do not establish antiviral protection, dosage, or safety in humans.

Summary

Study

AP-PG was tested for induction of interferon-stimulated genes in macrophage-like cells.

Finding

Mx1, ISG15, and viperin increased alongside transient IFN-beta and enhanced STAT1 signaling.

Limit

Cell signaling results do not establish antiviral effectiveness in people.

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Citation

Muramatsu D., Kawata K., Aoki S., Uchiyama H., Okabe M., Hara M., Inagaki N.. Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines. Scientific Reports. 2014. DOI: 10.1038/srep04777.

Study Details

Study Type

In Vitro

Published

2014

Journal

Scientific Reports

Authors

Muramatsu D., Kawata K., Aoki S., Uchiyama H., Okabe M., Hara M., Inagaki N.

DOI

10.1038/srep04777

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