Molecular cloning and expression of an isomalto-dextranase gene from Arthrobacter globiformis T6

This microbiology study cloned the imd gene encoding extracellular isomalto-dextranase from Arthrobacter globiformis T6 and expressed it in Escherichia coli. The gene contained a 1,926-base-pair open reading frame encoding a signal peptide and a mature enzyme, and transformed E. coli produced the enzyme in the periplasmic space under the gene's own promoter. The predicted protein showed no substantial sequence similarity to previously reported carbohydrases. This is foundational enzyme and gene-expression research; it does not evaluate beta-glucan supplementation, immunity, or health outcomes.