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In Vitro 2017

β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells

Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J. · International Journal of Biological Macromolecules · DOI: 10.1016/j.ijbiomac.2016.10.048
Read Original Paper DOI: 10.1016/j.ijbiomac.2016.10.048
Research Archive
TL;DR — Key Findings
  • Beta-1,3/1,6-glucans from two Diaporthe strains were characterized.
  • The preparations reduced viability in cultured breast- and liver-cancer cell lines under some conditions.
  • The findings are in vitro and do not demonstrate treatment effects or AP-glucan activity.

Abstract

This laboratory study purified high-molecular-weight beta-1,3/1,6-glucans secreted by two Diaporthe endophytes. Structural analysis identified a beta-1,3-linked backbone with glucose branches at the 1,6 position. The preparations reduced metabolic viability in cultured MCF-7 breast-cancer and HepG2-C3A liver-cancer cells under some test conditions. The work concerns Diaporthe glucans, not Aureobasidium beta-glucan, and measures short-term cell responses. It does not demonstrate tumor treatment, immune stimulation, antioxidant effects, or safety in animals or humans.

Summary

Summary

Study design

Two Diaporthe beta-glucans were purified, structurally characterized, and tested in two cancer-cell lines.

Interpretation

Reduced cell viability in vitro does not establish anticancer treatment efficacy, and the glucans were not from Aureobasidium.

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Citation

Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J.. β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells. International Journal of Biological Macromolecules. 2017. DOI: 10.1016/j.ijbiomac.2016.10.048.

Study Details
Study Type
In Vitro
Published
2017
Journal
International Journal of Biological Macromolecules
Authors
Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J.
DOI
10.1016/j.ijbiomac.2016.10.048
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