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Review 2017

β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells

Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J. · International Journal of Biological Macromolecules · DOI: 10.1016/j.ijbiomac.2016.10.048
Read Original Paper DOI: 10.1016/j.ijbiomac.2016.10.048
Research Archive
TL;DR — Key Findings
  • Diaporthe sp. produces β-(1→3,1→6)-d-glucan with immunostimulatory and antioxidant properties.
  • The β-1,3 backbone with β-1,6 branching confirmed as the key structural motif for bioactivity.
  • Shared structural features with AP-glucan reinforce the β-1,3/1,6 linkage as essential for immune activity.

Abstract

β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells

Highlights

  • β-glucans (EPS-P) were purified from the fermentation broth of two Diaporthe endophytes.
  • β-glucans consisted of a main chain of glucopyranosyl (1 → 3) linkages substituted at O-6 by glucosyl residues.
  • EPS-PD2 inhibited up to ∼75% of the proliferation of MCF-7 cells.
  • Both glucans inhibited up to ∼84% of the proliferation of HepG2-C3A cells.

Abstract

This study reports the characterization and antiproliferative activity of exopolysaccharides (EPS) produced by submerged cultures of the endophytes Diaporthe sp. JF766998 and Diaporthe sp. JF767007 isolated from the medicinal plant Piper hispidum Sw. Both strains secreted a crude EPS that, upon size exclusion chromatography, showed to contain a heteropolysaccharide (galactose, glucose and mannose) and a high-molecular weight glucan. Data from methylation analysis, FTIR and NMR spectroscopy (1H, COSY, TOCSY and HSQC-DEPT) indicated that the purified glucan consisted of a main chain of glucopyranosyl β-(1 → 3) linkages substituted at O-6 by glucosyl residues. According to MTT assay, some treatments of both β-glucans have antiproliferative activity against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells after 24 and 48 h of treatment, exhibiting a degree of inhibition ratio that reached the highest val

Summary

Summary

Background

This study investigated β-(1→3,1→6)-d-glucans produced by Diaporthe sp. endophytic fungi, examining production, structure, and biological activities of glucans from this underexplored source.

Key Findings

Diaporthe sp. produced β-(1→3,1→6)-d-glucans with immunostimulatory and antioxidant properties. The structural analysis confirmed β-1,3 backbone with β-1,6 branching — the same linkage pattern as other highly bioactive fungal β-glucans. Biological activity testing demonstrated macrophage activation and free radical scavenging capacity.

Relevance to Aureobasidium Beta Glucan Research

The shared β-(1→3,1→6) structural motif between Diaporthe and Aureobasidium pullulans β-glucans, combined with similar biological activities, supports the importance of this specific glucan linkage for immune activity. It reinforces AP-glucan's place within the broader family of biologically active β-1,3/1,6-glucans.

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Citation

Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J.. β-(1 → 3,1 → 6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells. International Journal of Biological Macromolecules. 2017. DOI: 10.1016/j.ijbiomac.2016.10.048.

Study Details
Study Type
Review
Published
2017
Journal
International Journal of Biological Macromolecules
Authors
Orlandelli R., Corradi da Silva M., Vasconcelos A., Magalhaes A., Telles M., Filho J., Pamphile J.
DOI
10.1016/j.ijbiomac.2016.10.048
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