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In Vitro 2021

Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay

Mukhametova, L. I.; Krylov, V. B.; Solovev, A. S.; Yashunsky, D. V.; Matveev, A. L.; Tikunova, N. V.; Eremin, S. A.; Nifantiev, N. E. · Russian Chemical Bulletin · DOI: 10.1007/s11172-021-3175-6
Read Original Paper DOI: 10.1007/s11172-021-3175-6
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TL;DR — Key Findings
  • Antibody 3G11 binding to a synthetic beta-1,3-glucan fragment was measured.
  • The result may help develop sensitive fungal beta-glucan detection assays.
  • This was an analytical method study, not a treatment study.

Abstract

This analytical study characterized the binding of monoclonal antibody 3G11 to a synthetic beta-1,3-glucan oligosaccharide. Fluorescence-polarization immunoassay was used to estimate the interaction in solution. The work was intended to support development of sensitive assays for detecting the low concentrations of fungal beta-1,3-glucan that may occur in biological samples. It concerns antibody affinity and measurement methodology; it does not evaluate beta-glucan supplementation, disease treatment, or patient outcomes.

Summary

Summary

Study design

A fluorescence-polarization immunoassay measured binding between antibody 3G11 and a synthetic beta-1,3-glucan fragment.

Interpretation

The study supports assay development and provides no evidence about therapeutic effects.

AI-generated summary for accessibility. Always refer to the original paper.

Citation

Mukhametova, L. I.; Krylov, V. B.; Solovev, A. S.; Yashunsky, D. V.; Matveev, A. L.; Tikunova, N. V.; Eremin, S. A.; Nifantiev, N. E.. Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay. Russian Chemical Bulletin. 2021. DOI: 10.1007/s11172-021-3175-6.

Study Details
Study Type
In Vitro
Published
2021
Journal
Russian Chemical Bulletin
Authors
Mukhametova, L. I.; Krylov, V. B.; Solovev, A. S.; Yashunsky, D. V.; Matveev, A. L.; Tikunova, N. V.; Eremin, S. A.; Nifantiev, N. E.
DOI
10.1007/s11172-021-3175-6
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