Highlights Abstract Polysaccharides from extracellular (PSEX), cell wall (PSCW), and intracellular (PSIN) fractions of Aureobasidium pullulans CGMCC19650 were extracted and purified, respectively. Based on chemical composition analysis and spectral characterization using Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, it was confirmed that the three polysaccharides are β-1,3-1,6-glucans. However, results of molecular weight analysis, Congo red